I missed helping all the little sisters. I hope this doesn't come back and bite me. I was near the 1 hour mark so I stopped playing. Stuck on a lock to a safe.
Alright! It was tough as I had a hunter alien on my heels the whole time, but I was able to reach Nicole, who was attached to the Marker. I defeated her (eventually) and made my escape with Ellie. What a cool game. Final boss and game ending! Great stream!